美國FOCUS單皰疹試劑盒

單皰疹1/2型免疫印跡法試劑盒（美國FOCUS）

TEST PROCEDURE
1.  Bring all reagents to room temperature before use. Remove the Antigen Well packet from cold storage. To avoid condensation, allow micro-well strips to
reach room temperature before opening the foil packet. If less than a full plate is to be used, return unused strips to the foil packet with desiccant and reseal
compley. Store unused antigen wells at 2 to 8°C. (Note: At the end of the assay, retain the frame for use with the remaining strips.)
2.  OPTIONAL 5 minute Pre-Soak. If omitting pre-soak, proceed to Step 3.
Fill wells with 1X Wash Buffer and soak for 5 minutes. Decant (or aspirate) the Antigen Wells and tap vigorously to remove 1X Wash Buffer. Blot the
emptied Antigen Wells face down on clean paper towels or absorbent paper to remove residual 1X Wash Buffer.
3.  Dispense 100 µL of the Sample Diluent into the “blank” well and 100 µL of each diluted specimen, control or calibrator (see Specimen, Controls, and
Calibrator Preparation, above) into the appropriate wells. (Note: For runs with more than 48 wells it is recommended that 250 µL of each diluted sample
first be added to a blank microtiter plate in the location corresponding to that in the ELISA wells. The samples can then be efficiently transferred into the
Antigen Wells with a 100 µL 8 or 12-channel pipettor.)
4.  Cover plates with sealing tape (or place in a humid chamber), and incubate for 60 ± 1 minute at room temperature (20 to 25°C).
5.  Remove sealing tape (or remove wells from the humid chamber), and empty the contents of the wells into a sink or a discard basin.
6.  Fill each well with a gentle stream of 1X Wash Buffer solution from a wash bottle then empty contents into a sink or a discard basin.
7.  Repeat wash (step 6) an additional 2 times.
8.  Tap the antigen wells vigorously to remove 1X Wash Buffer. Blot the emptied Antigen Wells face down on clean paper towels or absorbent paper to
remove residual 1X Wash Buffer.
9.  Dispense 100 µL Conjugate to all wells, using a 100 µL 8 or 12-channel pipettor.
10. Cover plates with sealing tape (or place in a humid chamber) and incubate for 30 ± 1 minutes at room temperature (20 to 25°C).
11. Repeat wash steps 5 through 8.
12. Pipet 100 µL of Substrate Reagent to all wells, using a 100 µL 8 or 12-channel pipettor. Begin incubation timing with the addition of Substrate Reagent to
the first well. (Note: Never pour the substrate reagent into the same trough as was used for the conjugate.)
13. Incubate for 10 ± 1 minutes at room temperature (20 to 25°C).
14. Stop the reaction by adding 100 µL of Stop Reagent to all wells using a 100 µL 8 or 12-channel pipettor. Add the Stop Reagent in the same sequence and at
the same pace as the Substrate was added. In antibody-positive wells, color should change from blue to yellow.
15. Gently blot the outside bottom of wells with a paper towel to remove droplets that may interfere with reading by the spectrophotometer. Do not rub with the
paper towel as it may scratch the optical surface of the well. (Note: Large bubbles on the surface of the liquid may affect the OD readings.)
16. Measure the absorbance of each well within 1 hour of stopping the assay. Set the microwell spectrophotometer at a wavelength of 450 nm. Zero the
instrument on the blank wells, or correct all ODs by manually subtracting the blank ODs.

單皰疹1/2型免疫印跡法試劑盒（美國FOCUS）

單純皰疹是一種由單純皰疹病毒所致的病毒性皮膚病，中醫稱為熱瘡。能引起人類多種疾病，如齦口炎（gingivostomatitis）、角膜結膜炎（keratoconjunctivitis）、腦炎（encephalitis）以及生殖系統感染和新生兒的感染。

廣州健侖生物公司提供以下單皰疹試劑盒：

ELISA IgG  單皰疹1酶免ELISA試劑盒FOCUS  96T

ELISA IgG  單皰疹2酶免ELISA試劑盒 FOCUS  96T

Immunoblot IgG 單皰疹1/2型免疫印跡法 24份/盒

更多詳細說明及原版英文說明書請工作人員。

【公司名稱】 廣州健侖生物科技有限公司
【】    楊永漢 
【】 
【騰訊 】 2042552662
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-3室